Rubin-Pitel Sheryl B, Luo Yunzi, Lee Jung-Kul, Zhao Huimin
Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Mol Biosyst. 2010 Aug;6(8):1444-6. doi: 10.1039/c004992a. Epub 2010 Jun 1.
Eucalyptus species synthesize a wealth of polyketide natural products, but no relevant biosynthetic enzyme has been identified. Degenerate primers designed from conserved regions of fourteen chalcone synthase superfamily enzymes were used to isolate gene fragments from at least five different Type III polyketide synthases (PKSs) in E. camaldulensis and E. robusta.