Mills T M, Stopper V S
Department of Physiology and Endocrinology, Medical College of Georgia, Augusta.
Lymphology. 1991 Mar;24(1):32-9.
A method is presented for the perfusion of rabbit ovaries in vitro which allows continuous collection of effluent perfusate from the ovarian vein and lymphatic system. The flow of ovarian lymph and the output of progesterone and 20 alpha dihydroprogesterone in lymph and venous effluent from perfused ovaries were measured and the results compared to the same parameters measured in vivo. Rates of flow of lymphatic and venous effluent and lymph/plasma protein ratios measured from perfused ovaries were similar to those measured in vivo, and were not statistically affected by the presence of corpora lutea in the ovaries. The concentrations of progesterone and 20 alpha dihydroprogesterone in ovarian venous blood/perfusate and lymph was increased by the presence of corpora lutea, but the concentration of progesterone was lower in vitro than in vivo. The concentration of these progestins in lymph suggest that only a small proportion of ovarian lymph is derived from corpora lutea in vitro or in vivo, and most is derived from ovarian interstitium.
本文介绍了一种体外灌注兔卵巢的方法，该方法可连续收集来自卵巢静脉和淋巴系统的流出灌注液。测量了卵巢淋巴流量以及灌注卵巢淋巴和静脉流出液中孕酮和20α-二氢孕酮的输出量，并将结果与体内测量的相同参数进行比较。从灌注卵巢测量的淋巴和静脉流出液流量以及淋巴/血浆蛋白比率与体内测量的相似，并且不受卵巢中黄体存在的统计学影响。黄体的存在会增加卵巢静脉血/灌注液和淋巴中孕酮和20α-二氢孕酮的浓度，但体外孕酮浓度低于体内。这些孕激素在淋巴中的浓度表明，体外或体内卵巢淋巴中只有一小部分来自黄体，大部分来自卵巢间质。
