[芒果苷对HL-60细胞周期状态及CDC2/细胞周期蛋白B1表达的影响]
[Effects of mangiferin on cell cycle status and CDC2/Cyclin B1 expression of HL-60 cells].
作者信息
Yao Yi-Bin, Peng Zhi-Gang, Liu Zhen-Fang, Yang Jie, Luo Jun
机构信息
Department of Hematology, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China.
出版信息
Zhong Yao Cai. 2010 Jan;33(1):81-5.
OBJECTIVE
To study the effects of mangiferin on cell cycle status and CDC2/Cyclin B1 expression in HL-60 cells, and its molecular mechanism for treating leukemia.
METHODS
The effect of Mangiferin on HL-60 cells proliferation was determined by MTT assay; The change of cell cycle status in HL-60 cells treated with mangiferin was performed by the flow cytometry; The expressions of CDC2 mRNA and Cyclin B1 mRNA were detected by semiquantitative RT-PCR.
RESULTS
The growth inhibition effects of mangiferin in HL-60 cells were enhanced as the mangiferin concentration increased and exposure time prolonged; HL-60 cells in G2/M phase increased in a dose-dependent way 24 hours after mangiferin administration, indicating G2/M phase blockage; the expressions of CDC2 mRNA and Cyclin B1 mRNA enhanced in a dose-dependent way and came to the peak at 80 micromol/L mangiferin.
CONCLUSION
Mangiferin can inhibit the proliferation of HL-60, block the cell cycle progression in G2/M phase, and it can significantly increase the expressions of CDC2 mRNA and Cyclin B1 mRNA of HL-60 cells. G2/M phase blockage may be one of its molecular mechanism for treating leukemia.
目的
研究芒果苷对HL-60细胞周期状态及CDC2/Cyclin B1表达的影响，及其治疗白血病的分子机制。
方法
采用MTT法检测芒果苷对HL-60细胞增殖的影响；应用流式细胞术检测芒果苷处理后HL-60细胞周期状态的变化；采用半定量RT-PCR检测CDC2 mRNA和Cyclin B1 mRNA的表达。
结果
芒果苷对HL-60细胞的生长抑制作用随其浓度增加和作用时间延长而增强；给药24小时后，G2/M期HL-60细胞呈剂量依赖性增加，提示出现G2/M期阻滞；CDC2 mRNA和Cyclin B1 mRNA的表达呈剂量依赖性增强，在芒果苷浓度为80 μmol/L时达到峰值。
结论
芒果苷可抑制HL-60细胞增殖，阻滞细胞周期于G2/M期，且可显著增加HL-60细胞CDC2 mRNA和Cyclin B1 mRNA的表达。G2/M期阻滞可能是其治疗白血病的分子机制之一。
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