[通过葡聚糖凝胶LH - 20柱纯化橄榄苦苷]
[Purification of oleuropein by sephadex LH-20 column].
作者信息
Dang Jian-Zhang, Nie Xiao-Zhong, Liang Li-Qin
机构信息
Applied Biological Department of Shenzhen Polytechnic College, Shenzhen 518055, China.
出版信息
Zhong Yao Cai. 2010 Jan;33(1):119-21.
OBJECTIVE
To purify the oleuropein crude extracts by sephadex LH-20 column chromatograph.
METHODS
This experiment used fast protein chromatography system (AKTA FPLC) produced by Amersham, Sweden. The chromatography column (20 mm x 300 mm) was matched with protein purification instrument. Sephadex LH-20 was used in the Fast Protein Liquid Chromatography columns. The mobile phase was 50% ethanol with a flow velocity of 1.0 mL per minute and the detection wavelength was 254 nm. The content of oleuropein was determined by HPLC.
RESULTS
The purity of oleuropein was 82.9% after passing the column twice when the sample volume was 2 mL.
CONCLUSION
Sephadex LH-20 can be re-used and the regeneration is convenient, it also provides a reference for the production of oleuropein.
目的
采用葡聚糖凝胶LH - 20柱色谱法纯化橄榄苦苷粗提物。
方法
本实验采用瑞典安玛西亚公司生产的快速蛋白质色谱系统（AKTA FPLC）。色谱柱（20mm×300mm）与蛋白质纯化仪配套。在快速蛋白质液相色谱柱中使用葡聚糖凝胶LH - 20。流动相为50%乙醇，流速为每分钟1.0mL，检测波长为254nm。采用高效液相色谱法测定橄榄苦苷的含量。
结果
当进样量为2mL时，经过两次过柱后，橄榄苦苷的纯度为82.9%。
结论
葡聚糖凝胶LH - 20可重复使用且再生方便，为橄榄苦苷的生产提供了参考。
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