Li Peizhi, Ren Junle, An Ting, Liu Yan
School of Life Science, Southwest University, Chongqing 400715, China.
Sheng Wu Gong Cheng Xue Bao. 2010 Mar;26(3):324-9.
Cytoplasmic transduction peptide (CTP) is a newly designed transduction peptide, by which special molecules can be carried out and localized into cytoplasmic compartment. Superoxide dismutase (SOD) is a protein that is difficult to go into cytoplasm. In this study, CTP-SOD fusion gene was amplified from human cDNA by PCR, and the active recombinant protein was successfully expressed in Pichia pastoris. HeLa cells pretreated with CTP-SOD showed a significantly improved survival against the pyrogallol-induced oxidative stress, suggesting CTP-SOD could cross the cell membrane more efficiently and protect cells from oxidative stress.
细胞质转导肽（CTP）是一种新设计的转导肽，通过它可以携带特殊分子并将其定位到细胞质区室中。超氧化物歧化酶（SOD）是一种难以进入细胞质的蛋白质。在本研究中，通过PCR从人cDNA中扩增出CTP-SOD融合基因，并在毕赤酵母中成功表达了活性重组蛋白。用CTP-SOD预处理的HeLa细胞在面对邻苯三酚诱导的氧化应激时存活率显著提高，这表明CTP-SOD可以更有效地穿过细胞膜并保护细胞免受氧化应激。
