National Institute of Advanced Industrial Science and Technology (AIST), Ikeda, Osaka, Japan.
J Microbiol Biotechnol. 2010 May;20(5):889-92. doi: 10.4014/jmb.0912.12020.
We investigated a potential for glucose production from cellulose material using two kinds of hyperthermophilic enzymes, endo-cellulase (EG) and beta-glucosidase (BGL). Two BGLs from hyperthermophile Pyrococcus furiosus and mesophile Aspergillus aculeatus were compared for complete hydrolysis of cellulose with P. horikoshii endo-cellulase (EGPh). The combination reactions by each BGL enzyme and EGPh could produce only glucose without the other oligosaccharides from phosphoric acid swollen Avicel (PSA). The combination of the both hyperthermophilic cellulases, BGLPf and EGPh, will be adaptable to high efficient system to produce glucose at high temperature.
我们研究了使用两种嗜热酶——内切纤维素酶（EG）和β-葡萄糖苷酶（BGL）从纤维素材料中生产葡萄糖的潜力。比较了两种来自嗜热菌 Pyrococcus furiosus 和中温菌 Aspergillus aculeatus 的 BGL，以与来自 Pyrococcus horikoshii 的内切纤维素酶（EGPh）一起完全水解纤维素。每个 BGL 酶和 EGPh 的组合反应只能从磷酸膨胀的 Avicel（PSA）中产生葡萄糖而没有其他寡糖。两种嗜热纤维素酶，BGLPf 和 EGPh 的组合，将适应高温下高效生产葡萄糖的系统。
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