Komura J, Mitani H, Nemoto N, Ishikawa T, Shima A
Zoological Institute, Faculty of Science, University of Tokyo, Japan.
Mutat Res. 1991 May;254(3):191-8. doi: 10.1016/0921-8777(91)90056-u.
The time courses of excision repair and photoreactivation of pyrimidine dimers induced by 254-nm UV were examined in the genome overall and in the c-ras sequence of RBCF-1 cells derived from a goldfish, by the use of UV endonuclease of Micrococcus luteus and alkaline agarose gel electrophoresis. Excision repair was more efficient in the ras sequence than in the genome overall, whereas no differences in efficiency of photoreactivation were detected. These results suggest that excision repair is affected by the accessibility of chromatin, while photoreactivation is not.
利用藤黄微球菌的紫外线内切酶和碱性琼脂糖凝胶电泳技术,研究了254纳米紫外线诱导的嘧啶二聚体在金鱼来源的RBCF - 1细胞的整个基因组和c - ras序列中的切除修复和光复活的时间进程。切除修复在ras序列中比在整个基因组中更有效,而光复活效率未检测到差异。这些结果表明,切除修复受染色质可及性的影响,而光复活则不受影响。