Freeman S E
Division of Biomedical Research, Lovelace Medical Foundation, Albuquerque, New Mexico 87108.
J Invest Dermatol. 1988 Jun;90(6):814-7. doi: 10.1111/1523-1747.ep12462039.
The excision repair kinetics of UVB (280-320 nm)*-induced pyrimidine dimers in DNA of human skin in situ was determined for seventeen volunteers using a dimer-specific endonuclease from Micrococcus luteus in conjunction with agarose gel electrophoresis. Removal of pyrimidine dimers from human skin could be detected within 6 h after irradiation and the average half-life for removal of pyrimidine dimers was 11.0 h (+/- 4.3 h). However, there was significant inter-individual variability of repair as indicated by a half-life coefficient of variation of 38%.
使用来自藤黄微球菌的二聚体特异性核酸内切酶结合琼脂糖凝胶电泳，测定了17名志愿者原位人皮肤DNA中UVB（280 - 320纳米）诱导的嘧啶二聚体的切除修复动力学。照射后6小时内可检测到从人皮肤中去除嘧啶二聚体，去除嘧啶二聚体的平均半衰期为11.0小时（±4.3小时）。然而，修复存在显著的个体间差异，半衰期变异系数为38%即表明了这一点。
