Ocular Tissue Bank General Teaching Hospital, Charles University, Prague, Czech Republic.
Cornea. 2010 Sep;29(9):996-9. doi: 10.1097/ICO.0b013e3181cc7b37.
The aim of this study was to assess the presence of pathogenic prions in the brain tissue of eye donors and to evaluate the benefits of 2-year obligatory testing in the Czech Republic.
Brain tissue was retrieved during autopsies of eye donors of 3 tissue banks in the Czech Republic. The frozen specimens obtained from the frontal lobe were transported to the Czech National Reference Laboratory for the diagnosis of human prion disorders. The presence of pathogenic prions was tested using the Prionics-Check WESTERN kit. Confirmative Western blotting using 1 of 2 different clones of monoclonal anti-PrP antibody was performed as well.
No pathogenic prions were found in any of the 1142 tested specimens. One specimen revealed weak positivity at initial screening; however, repeated examination of the specimen and other specimens from different locations in the brain of the same donor did not confirm the presence of pathogenic prions. The negative result was confirmed by the National CJD Surveillance Unit, University of Edinburgh, United Kingdom.
The absence of pathogenic prions from all of the 1142 tested specimens corresponds to the presumed very low risk of transmission of Creutzfeldt-Jakob disease through corneal graft transplantation. As a result of this disorder's rarity, a larger series of tested samples should be evaluated to obtain statistically significant findings. Although such testing increases the safety of donor eye tissue, it also increases the expense, causes organizational difficulties, and may extend the time needed to release the tissue for grafting.
本研究旨在评估眼供体脑组织中是否存在致病性朊病毒，并评估在捷克共和国进行 2 年强制性检测的益处。
从捷克共和国的 3 个组织库的眼供体尸检中获取脑组织。从额叶获得的冷冻标本被运送到捷克国家人类朊病毒疾病诊断参考实验室。使用 Prionics-Check WESTERN 试剂盒检测致病性朊病毒的存在。还进行了使用 2 种单克隆抗 PrP 抗体的 1 种不同克隆的确认性 Western 印迹。
在 1142 个测试标本中均未发现致病性朊病毒。一个标本在初始筛选时显示弱阳性；然而，对该标本和同一供体大脑中不同部位的其他标本进行重复检查并未确认致病性朊病毒的存在。英国爱丁堡大学国家 CJD 监测单位也证实了阴性结果。
从所有 1142 个测试标本中均未发现致病性朊病毒，这与通过角膜移植传播克雅氏病的假定极低风险相符。由于该疾病的罕见性，应该评估更大系列的测试样本以获得具有统计学意义的发现。尽管这种检测增加了供体眼组织的安全性，但也增加了费用、造成组织困难，并可能延长用于移植的组织释放时间。
