鉴定抗狂犬病毒核蛋白构象特异性单克隆抗体。
Characterization of conformation-specific monoclonal antibodies against rabies virus nucleoprotein.
机构信息
Department of Pathology, College of Veterinary Medicine, University of Georgia, 501 D.W. Brooks Drive, Athens, GA 30602, USA.
出版信息
Arch Virol. 2010 Aug;155(8):1187-92. doi: 10.1007/s00705-010-0709-x. Epub 2010 Jun 4.
Abstract
Three anti-rabies virus (RABV) nucleoprotein (N) monoclonal antibodies (Mab) were characterized by immunofluorescence assays, western blotting, and immunohistochemistry. One of these Mabs recognized the antigen by all of the assays, while the other two recognized N only in the native form in the immunofluorescence assay. These data, together with epitope mapping studies, suggest that two anti-N Mabs recognize conformational epitopes located within the N-terminal region of the RABV N protein. The availability of Mabs specific for both linear and epitope-specific antibodies should prove valuable for rabies diagnosis as well as for RABV N protein structure-function studies.
摘要
三种抗狂犬病毒（RABV）核蛋白（N）单克隆抗体（Mab）通过免疫荧光分析、免疫印迹和免疫组织化学进行了表征。其中一种 Mab 通过所有检测方法识别抗原，而另外两种 Mab 仅在免疫荧光分析中的天然形式下识别 N。这些数据，加上表位作图研究，表明两种抗 N Mab 识别位于 RABV N 蛋白 N 端区域内的构象表位。针对线性和表位特异性抗体的 Mab 的可用性，对于狂犬病诊断以及 RABV N 蛋白结构-功能研究，应该具有重要的价值。
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