出乎意料的是，雌雄异株的 Honckenya peploides（石竹科）大型单性群中存在着高度的遗传变异。

Unexpectedly high genetic variation in large unisexual clumps of the subdioecious plant Honckenya peploides (Caryophyllaceae).

机构信息

Area de Ecología, Facultad de Biología, Avda. Lope Gómez de Marzoa s/n, Universidad de Santiago de Compostela, Santiago de Compostela, Spain.

出版信息

Plant Biol (Stuttg). 2010 May 1;12(3):518-25. doi: 10.1111/j.1438-8677.2009.00231.x.

DOI:10.1111/j.1438-8677.2009.00231.x

PMID:20522189

Abstract

Honckenya peploides is a subdioecious dune plant that reproduces both sexually and by clonal growth. In northwest Spain this species was found to exhibit an extreme spatial segregation of the sexes, and our objective was to investigate genetic variation in unisexual clumps. Genetic variation was studied in six unisexual clumps of H. peploides, three of them exclusively composed of males and three exclusively female. In total, 193 samples were analysed using isozyme analysis and 80 samples were analysed using two AFLP primer combinations. Both techniques revealed considerably high genetic diversity (average proportion of distinguishable genotypes: 0.22 for isozymes and 0.36 for AFLP; average Simpson's D: 0.65 for isozymes and 0.68 for AFLP). Our results show that, in spite of clonal growth, each unisexual clump consists of different genotypes. Genetic diversity within clumps is similar for both sexual morphs. Reasons for unisexuality of the clumps are discussed.

摘要

海石竹是一种雌雄异株的沙丘植物，既能有性繁殖也能通过克隆生长进行繁殖。在西班牙西北部，发现该物种的性别表现出极端的空间分离，我们的目标是研究单性克隆的遗传变异。对 6 个单性海石竹克隆体进行了遗传变异研究，其中 3 个克隆体仅由雄性组成，3 个克隆体仅由雌性组成。总共分析了 193 个样本的同工酶分析和 80 个样本的两种 AFLP 引物组合分析。两种技术均显示出相当高的遗传多样性（可区分基因型的平均比例：同工酶为 0.22，AFLP 为 0.36；Simpson's D 平均：同工酶为 0.65，AFLP 为 0.68）。我们的结果表明，尽管克隆生长，但每个单性克隆体都由不同的基因型组成。两种性形态的克隆内遗传多样性相似。讨论了克隆单性的原因。