吉西他滨联合乳香引起胰腺癌细胞强烈的生长抑制和凋亡。
Gemcitabine combined with gum mastic causes potent growth inhibition and apoptosis of pancreatic cancer cells.
机构信息
Department of Surgery, the Sixth People's Hospital, Shanghai Jiaotong University, Shanghai, China.
出版信息
Acta Pharmacol Sin. 2010 Jun;31(6):741-5. doi: 10.1038/aps.2010.54.
AIM
To investigate the antiproliferative and apoptotic effects of gemcitabine combined with gum mastic and the underlying mechanisms in human pancreatic cancer cell lines.
METHODS
Cell proliferation and apoptosis were examined using the methyl thiazolyl tetrazolium (MTT) assay and propidium iodine staining, respectively. The expression of Bcl-2, Bax, NF-kappaB p65 subunit, and IkappaBalpha protein was measured using Western blotting.
RESULTS
Gemcitabine 0.01-100 microg/mL inhibited cell proliferation and induced apoptosis in both pancreatic cancer BxPC-3 and COLO 357 cells. Gum mastic 40 microg/mL significantly potentiated the antiproliferative and apoptotic effects of gemcitabine 10 microg/mL after 72-h treatment. When cells were treated with gemcitabine in combination with gum mastic, the IkappaBalpha level was increased, whereas NF-kappaB activation was blocked; the expression of Bax protein was substantially increased, but Bcl-2 protein was down-regulated.
CONCLUSION
Gemcitabine combined with gum mastic causes potent apoptosis in pancreatic cancer cells. The combination may be an effective therapeutic strategy for pancreatic cancer.
目的
研究吉西他滨联合乳香对人胰腺癌细胞系的增殖抑制和凋亡作用及其机制。
方法
采用噻唑蓝（MTT）比色法和碘化丙啶染色法检测细胞增殖和凋亡，Western blot 法检测 Bcl-2、Bax、NF-κB p65 亚单位和 IκBα蛋白的表达。
结果
吉西他滨 0.01-100μg/ml 抑制胰腺癌细胞 BxPC-3 和 COLO 357 的增殖并诱导其凋亡。乳香 40μg/ml 与吉西他滨 10μg/ml 联合作用 72h 后，明显增强了吉西他滨的增殖抑制和凋亡作用。吉西他滨联合乳香作用后，细胞 IκBα水平增加，NF-κB 激活被阻断；Bax 蛋白表达明显增加，而 Bcl-2 蛋白下调。
结论
吉西他滨联合乳香能诱导胰腺癌细胞发生强烈的凋亡。该联合治疗可能为胰腺癌的治疗提供一种有效的策略。
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