CIMMYT, Int. Apdo. Postal 6-641, 06600, Mexico DF, Mexico.
Theor Appl Genet. 2010 Oct;121(6):1001-21. doi: 10.1007/s00122-010-1351-4. Epub 2010 Jun 4.
A restricted range in height and phenology of the elite Seri/Babax recombinant inbred line (RIL) population makes it ideal for physiological and genetic studies. Previous research has shown differential expression for yield under water deficit associated with canopy temperature (CT). In the current study, 167 RILs plus parents were phenotyped under drought (DRT), hot irrigated (HOT), and temperate irrigated (IRR) environments to identify the genomic regions associated with stress-adaptive traits. In total, 104 QTL were identified across a combination of 115 traits × 3 environments × 2 years, of which 14, 16, and 10 QTL were associated exclusively with DRT, HOT, and IRR, respectively. Six genomic regions were related to a large number of traits, namely 1B-a, 2B-a, 3B-b, 4A-a, 4A-b, and 5A-a. A yield QTL located on 4A-a explained 27 and 17% of variation under drought and heat stress, respectively. At the same location, a QTL explained 28% of the variation in CT under heat, while 14% of CT variation under drought was explained by a QTL on 3B-b. The T1BL.1RS (rye) translocation donated by the Seri parent was associated with decreased yield in this population. There was no co-location of consistent yield and phenology or height-related QTL, highlighting the utility of using a population with a restricted range in anthesis to facilitate QTL studies. Common QTL for drought and heat stress traits were identified on 1B-a, 2B-a, 3B-b, 4A-a, 4B-b, and 7A-a confirming their generic value across stresses. Yield QTL were shown to be associated with components of other traits, supporting the prospects for dissecting crop performance into its physiological and genetic components in order to facilitate a more strategic approach to breeding.
高度和生育期范围受限的精英 Seri/Babax 重组自交系（RIL）群体非常适合进行生理和遗传研究。之前的研究表明，与冠层温度（CT）相关的水分亏缺下产量存在差异表达。在当前的研究中，对 167 个 RIL 及其亲本在干旱（DRT）、热灌溉（HOT）和温和灌溉（IRR）环境下进行了表型分析，以鉴定与应激适应性状相关的基因组区域。总共在 115 个性状×3 个环境×2 年的组合中鉴定到 104 个 QTL，其中 14、16 和 10 个 QTL 分别与 DRT、HOT 和 IRR 完全相关。6 个基因组区域与大量性状相关，分别是 1B-a、2B-a、3B-b、4A-a、4A-b 和 5A-a。位于 4A-a 的一个产量 QTL 分别解释了干旱和热胁迫下变异的 27%和 17%。在同一位置，一个 QTL 解释了热胁迫下 CT 变异的 28%，而干旱下 CT 变异的 14%是由 3B-b 上的一个 QTL 解释的。Seri 亲本提供的 T1BL.1RS（黑麦）易位与该群体产量降低有关。在花期和生育期或高度相关 QTL 中没有一致的产量和位置，这突出了使用开花范围受限的群体来促进 QTL 研究的效用。在 1B-a、2B-a、3B-b、4A-a、4B-b 和 7A-a 上鉴定到了干旱和热胁迫性状的共同 QTL，证实了它们在各种胁迫下的通用价值。产量 QTL 与其他性状的组成部分相关，这支持了将作物性能分解为生理和遗传成分的前景，以便更有策略地进行育种。
