Genome Biotechnology Laboratory, Kanazawa Institute of Technology, 3-1 Yatsukaho, Hakusan, Ishikawa 924-0838, Japan.
J Biomed Mater Res B Appl Biomater. 2010 Jul;94(1):53-63. doi: 10.1002/jbm.b.31623.
If endothelial progenitor cells (EPCs), which circulate in blood flow, are captured on the luminal surface of an implanted artificial graft and sooner or later proliferate to form a fully endothelialized surface, such a small-diameter artificial graft must exhibit a high patency rate. This study aimed at designing a luminal surface of elastomeric electrospun mesh graft, which is capable of selective capture of EPCs under arterial flow and has a high antithrombogenic potential until full endothelization is achieved. The designed luminal surface layer is composed of a photopolymerized gelatin gel layer that enables the release of impregnated heparin and selective adhesion of circulating EPCs via complexation between surface-fixed vascular endothelial growth factor (VEGF) and cellular VEGF receptor. Human mononuclear cells seeded and cultured on such a gel layer expressed endothelial cell surface markers. Confocal laser scanning microscopy observation revealed that VEGF is highly surface-enriched, and heparin is homogeneously distributed in the gel layer. A continuously slow release of heparin was observed. Thus, a prototype luminal surface was fabricated on electrospun segmented polyurethane tubes for in vivo study.
如果循环血流中的内皮祖细胞 (EPCs) 能够黏附在植入的人工移植物的管腔表面，并在一定时间后增殖形成完全内皮化的表面，那么这种小直径人工移植物就必须具有高通畅率。本研究旨在设计一种弹性体电纺网人工移植物的管腔表面，使其能够在动脉血流下选择性捕获 EPCs，并且具有高抗血栓形成潜力，直到完全内皮化。设计的管腔表面层由光聚合明胶凝胶层组成，该凝胶层能够通过表面固定的血管内皮生长因子 (VEGF) 与细胞 VEGF 受体之间的络合作用，释放浸渍的肝素并选择性黏附循环中的 EPCs。在这样的凝胶层上接种和培养的人单核细胞表达内皮细胞表面标志物。共聚焦激光扫描显微镜观察显示，VEGF 高度表面富集，肝素在凝胶层中均匀分布。观察到肝素持续缓慢释放。因此，在体内研究中，在电纺分段聚氨酯管上制造了原型管腔表面。
