Sobczak Agnieszka, Zajac Marianna, Malinka Wiesław, Redzicka Aleksandra
Department of Pharmaceutical Chemistry, Poznan University of Medical Sciences, Grunwaldzka 6, 60-780 Poznań, Poland.
Acta Pol Pharm. 2010 May-Jun;67(3):225-32.
The stability of N-[2-(4-o-fluorophenylpiperazin-1-yl)ethyl]-2,5-dimethyl-1-phenylpyrrole-3,4-dicarboximide (PDI; a derivative with an analgesic activity) was studied in order to investigate its degradation mechanism and identify its degradation products in aqueous-organic solutions. The stability of PDI and its two degradation products (A and B) was performed with an HPLC method: (LiChrospher C18 column (250 x 4 mm LD., dp = 5 microm), mobile phase: 3.5 g/L solution of sodium lauryl sulfate and 1.6 mL of phosphoric acid(V)-acetonitrile (40:60, v/v), UV detector: 240 nm, flow rate: 1 ml/min). The identification of products A and B was conducted using HPLC-ES-MS and 1H- and 13C-NMR methods.
