豚鼠血管活性肠肽（VIP）与普通VIP对豚鼠和大鼠肝肺膜以及人淋巴细胞性SUP-T1膜的体外比较效应。

Comparative in vitro effects of guinea pig VIP and common VIP on liver and lung membranes from guinea pig and rat and on human lymphoblastic SUP-T1 membranes.

作者信息

Cauvin A, Buscail L, Gourlet P, De Neef P, Robberecht P, Yanaihara N, Christophe J

机构信息

Department of Biochemistry and Nutrition, Medical School, Université Libre de Bruxelles, Brussels, Belgium.

出版信息

Peptides. 1991 Jan-Feb;12(1):139-43. doi: 10.1016/0196-9781(91)90180-w.

DOI:10.1016/0196-9781(91)90180-w

PMID:2052490

Abstract

Guinea pig VIP differs from VIP of several mammals by its amino acids in positions 5, 9, 19 and 26. We tested a) its ability to occupy VIP receptors in liver and lung membranes of rat and guinea pig and in the human lymphoblastic SUP-T1 cell line and b) the ensuing adenylate cyclase stimulation. In liver and lung membranes from rat, guinea pig VIP was less potent than common VIP to occupy high and low affinity VIP receptors. In rat liver both VIP activated adenylate cyclase mostly through high affinity receptors. In rat lung, guinea pig VIP activated the enzyme mostly through high affinity receptors and was less efficient than common VIP acting through both classes of receptors. In guinea pig liver and lung membranes, binding inhibition curves were steeper than with rat preparations and adenylate cyclase appeared to be mostly activated through high affinity VIP receptors in liver and through both classes of receptors in lung. On human lymphoblastic SUP-T1 membranes both VIP were equally potent and efficient to inhibit tracer binding and activate adenylate cyclase.

摘要

豚鼠血管活性肠肽（VIP）在第5、9、19和26位氨基酸上与几种哺乳动物的VIP不同。我们测试了：a）其占据大鼠和豚鼠肝和肺膜以及人淋巴细胞性SUP-T1细胞系中VIP受体的能力；b）随后的腺苷酸环化酶刺激作用。在大鼠肝和肺膜中，豚鼠VIP占据高亲和力和低亲和力VIP受体的能力比普通VIP弱。在大鼠肝脏中，两种VIP主要通过高亲和力受体激活腺苷酸环化酶。在大鼠肺中，豚鼠VIP主要通过高亲和力受体激活该酶，且比通过两类受体起作用的普通VIP效率低。在豚鼠肝和肺膜中，结合抑制曲线比大鼠制剂的更陡，腺苷酸环化酶似乎主要通过肝脏中的高亲和力VIP受体以及肺中的两类受体被激活。在人淋巴细胞性SUP-T1膜上，两种VIP在抑制示踪剂结合和激活腺苷酸环化酶方面同样有效。