Effect of freezing on the coupling of VIP receptors to adenylate cyclase in rat liver membranes.
作者信息
Robberecht P, Waelbroeck M, De Neef P, Camus J C, Gourlet P, Christophe J
机构信息
Department of Biochemistry and Nutrition, School of Medicine, Universit Libre de Bruxelles, Belgium.
出版信息
Life Sci. 1988;42(5):505-10. doi: 10.1016/0024-3205(88)90090-2.
PMID:2828794
Abstract
In fresh rat liver plasma membranes, high affinity VIP receptors were specifically labelled with [125I] helodermin and were well coupled to adenylate cyclase while low affinity VIP receptors were not. After freezing and thawing low affinity VIP receptors were also coupled to adenylate cyclase. This modification of adenylate cyclase activation was specific for the VIP response as freezing and thawing did not modify Gpp (NH)p, NaF and glucagon stimulations.
摘要
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