发夹 DNA 包覆的金纳米粒子作为细胞内 mRNA 探针用于检测黑色素瘤细胞中天冬氨酸酶基因的表达。
Hairpin DNA coated gold nanoparticles as intracellular mRNA probes for the detection of tyrosinase gene expression in melanoma cells.
机构信息
Department of Chemistry, Vanderbilt University, Station B351822, Nashville, TN 37235, USA.
出版信息
Chem Commun (Camb). 2010 Aug 14;46(30):5557-9. doi: 10.1039/c001969k. Epub 2010 Jun 4.
DOI:10.1039/c001969k
PMID:20526488
Abstract
A new intracellular mRNA imaging probe has been developed that incorporates thiol-terminated hairpin oligonucleotides covalently bound to the surface of citrate-capped gold nanoparticles. The hairpin DNA-coated gold nanoparticles (hAuNPs) positively identifies tyrosinase mRNA in cultured melanoma cells.
摘要
一种新的细胞内 mRNA 成像探针已经被开发出来，它将巯基终止的发夹寡核苷酸共价结合到柠檬酸封端的金纳米粒子表面。这种发夹 DNA 涂层的金纳米粒子（hAuNPs）可以在培养的黑色素瘤细胞中特异性识别酪氨酸酶 mRNA。
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