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发夹 DNA 包覆的金纳米粒子作为细胞内 mRNA 探针用于检测黑色素瘤细胞中天冬氨酸酶基因的表达。

Hairpin DNA coated gold nanoparticles as intracellular mRNA probes for the detection of tyrosinase gene expression in melanoma cells.

机构信息

Department of Chemistry, Vanderbilt University, Station B351822, Nashville, TN 37235, USA.

出版信息

Chem Commun (Camb). 2010 Aug 14;46(30):5557-9. doi: 10.1039/c001969k. Epub 2010 Jun 4.

DOI:10.1039/c001969k
PMID:20526488
Abstract

A new intracellular mRNA imaging probe has been developed that incorporates thiol-terminated hairpin oligonucleotides covalently bound to the surface of citrate-capped gold nanoparticles. The hairpin DNA-coated gold nanoparticles (hAuNPs) positively identifies tyrosinase mRNA in cultured melanoma cells.

摘要

一种新的细胞内 mRNA 成像探针已经被开发出来，它将巯基终止的发夹寡核苷酸共价结合到柠檬酸封端的金纳米粒子表面。这种发夹 DNA 涂层的金纳米粒子（hAuNPs）可以在培养的黑色素瘤细胞中特异性识别酪氨酸酶 mRNA。

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Hairpin DNA coated gold nanoparticles as intracellular mRNA probes for the detection of tyrosinase gene expression in melanoma cells.发夹 DNA 包覆的金纳米粒子作为细胞内 mRNA 探针用于检测黑色素瘤细胞中天冬氨酸酶基因的表达。
Chem Commun (Camb). 2010 Aug 14;46(30):5557-9. doi: 10.1039/c001969k. Epub 2010 Jun 4.
2
Applications of Hairpin DNA-Functionalized Gold Nanoparticles for Imaging mRNA in Living Cells.发夹DNA功能化金纳米颗粒在活细胞mRNA成像中的应用。
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Tyrosinase mRNA levels in the blood of uveal melanoma patients: correlation with the number of circulating tumor cells and tumor progression.葡萄膜黑色素瘤患者血液中酪氨酸酶 mRNA 水平与循环肿瘤细胞数量及肿瘤进展的相关性。
Melanoma Res. 2010 Aug;20(4):303-10. doi: 10.1097/CMR.0b013e32833906e3.
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Optimization of the tyrosinase mRNA probe to detect circulating melanocytes with reverse transcription and polymerase chain reaction.
Cell Mol Biol (Noisy-le-grand). 1998 Dec;44(8):1247-52.
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Cotransfection of genes encoding human tyrosinase and tyrosinase-related protein-1 prevents melanocyte death and enhances melanin pigmentation and gene expression of Lamp-1.共转染编码人酪氨酸酶和酪氨酸酶相关蛋白-1的基因可防止黑素细胞死亡，并增强黑素生成以及溶酶体相关膜蛋白-1的基因表达。
Exp Cell Res. 1994 Jul;213(1):231-41. doi: 10.1006/excr.1994.1195.
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Melanoma-specific expression in first-generation adenoviral vectors in vitro and in vivo -- use of the human tyrosinase promoter with human enhancers.第一代腺病毒载体在体外和体内的黑色素瘤特异性表达——人酪氨酸酶启动子与人增强子的应用。
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Detection of tyrosinase mRNA from the blood of melanoma patients.检测黑色素瘤患者血液中的酪氨酸酶信使核糖核酸。
Cancer Epidemiol Biomarkers Prev. 1996 Apr;5(4):293-6.
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Molecular detection of circulating tyrosinase mRNA: optimization in a preclinical xenograft mouse melanoma model and further evaluation in samples from advanced melanoma patients.
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The detection of tyrosinase mRNA in the peripheral blood of stage I melanoma patients is not of clinical relevance in predicting metastasis risk and survival.在I期黑色素瘤患者外周血中检测酪氨酸酶mRNA，对于预测转移风险和生存率并无临床意义。
Melanoma Res. 2000 Apr;10(2):113-8.
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The POU domain transcription factor Brn-2: elevated expression in malignant melanoma and regulation of melanocyte-specific gene expression.POU结构域转录因子Brn-2：在恶性黑色素瘤中表达升高及对黑素细胞特异性基因表达的调控
Oncogene. 1995 Nov 16;11(10):2157-64.

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