National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P.O. Box 353, Beijing 100190, People's Republic China.
Appl Biochem Biotechnol. 2010 Dec;162(8):2244-58. doi: 10.1007/s12010-010-8998-9. Epub 2010 Jun 6.
In this study, we evaluated the feasibility of solid-state fermentation (SSF) on polyurethane foam (PUF) for xanthan production. The effects of air pressure pulsation (APP) on biomass accumulation and final xanthan concentration were also studied. Under suitable conditions (15% inoculum, 0.5-cm (side length) PUF cubes, 15 mL medium per gram cubes and 4.5 cm bed depth), the broth was dispersed on the PUF as a film. When the initial glucose concentration in the media was low (20 and 40 g L⁻¹), there was no significant difference between the final xanthan concentration in static SSF and submerged fermentation (SMF). When high initial glucose concentrations (60 and 80 g L⁻¹) were used, the final gum concentrations in SSF were much higher than those in SMF. When the APP technique was applied in xanthan production with a medium containing a high glucose concentration (80 g L⁻¹), the oxygen consumption rate of Xanthomonas campestris was significantly enhanced at the later stages of fermentation, and both the biomass and xanthan concentration were improved. The results indicated that SSF on PUF is suitable for xanthan preparation, especially when the initial glucose concentration ranged from 60 to 80 g L⁻¹. Those results also demonstrated that APP technology can be used to enhance xanthan yields.
在这项研究中，我们评估了在聚氨酯泡沫（PUF）上进行固态发酵（SSF）生产黄原胶的可行性。还研究了空气压力脉动（APP）对生物量积累和最终黄原胶浓度的影响。在合适的条件下（15%接种量、0.5 厘米（边长）PUF 立方体、每克立方体 15 毫升培养基和 4.5 厘米床深），将发酵液分散在 PUF 上形成薄膜。当培养基中的初始葡萄糖浓度较低（20 和 40 g L⁻¹）时，静态 SSF 和浸没发酵（SMF）的最终黄原胶浓度没有显著差异。当使用较高的初始葡萄糖浓度（60 和 80 g L⁻¹）时，SSF 中的最终胶浓度远高于 SMF。当 APP 技术应用于含有高葡萄糖浓度（80 g L⁻¹）的培养基中的黄原胶生产时，在发酵后期黄单胞菌的耗氧率显著提高，生物量和黄原胶浓度都得到了提高。结果表明，PUF 上的 SSF 适用于黄原胶的制备，特别是当初始葡萄糖浓度在 60 到 80 g L⁻¹ 之间时。这些结果还表明，APP 技术可用于提高黄原胶的产量。
