Bregman M D, Cheng S, Levy D
Biochim Biophys Acta. 1978 Apr 3;539(4):489-95. doi: 10.1016/0304-4165(78)90082-x.
The reaction of glucagon with 4-fluoro-3-nitrophenylazide has been shown to afford the photosensitive derivative, Nepsilon-4-azido-2-nitrophenyl-glucagon. The structure and properties of this derivative were established by amino acid analysis, absorption and fluorescence spectroscopy, deamination, Edman degradation and photolysis. This photoaffinity derivative of glucagon has been used to label specifically glucagon binding sites on hepatocyte plasma membranes.
已证明胰高血糖素与4-氟-3-硝基苯叠氮化物反应可生成光敏衍生物Nε-4-叠氮基-2-硝基苯基-胰高血糖素。通过氨基酸分析、吸收光谱和荧光光谱、脱氨基作用、埃德曼降解和光解确定了该衍生物的结构和性质。这种胰高血糖素的光亲和衍生物已用于特异性标记肝细胞质膜上的胰高血糖素结合位点。
