Department of Biological Chemistry, Yamaguchi University, 1677-1 Yoshida, Yamaguchi-shi, Yamaguchi 753-8515, Japan.
J Agric Food Chem. 2010 Jul 14;58(13):7986-90. doi: 10.1021/jf100793d.
A major allergen of Japanese cedar, Cry j 1, was conjugated with galactomannan (M(w) of 15 kDa), dextran (M(w) of 12 kDa), xyloglucan (M(w) of 1.4 kDa), and various monosaccharides through the Maillard reaction by dry-heating in 65% relative humidity. The Cry j 1-galactomannan conjugate completely masked the epitopes of the allergen in Cry j 1. The Cry j 1-dextran conjugate also masked the epitopes of Cry j 1. The small size of oligosaccharide (xyloglucan) and various monosaccharides cannot mask the epitopes of allergen Cry j 1. This suggests that the higher molecular size of attached saccharides is important to mask sterically the epitope sites. The Cry j 1-galactomannan and Cry j 1-mannose conjugates were effectively trafficked in the gut and co-localized with immune cells, such as dendritic cells in the gut, suggesting that Cry j 1-saccharide conjugates are phagocytosed via the mannose receptor in immune cells. These results suggest that the Cry j 1-galactomannan conjugate is suitable for masking the epitope sites of Cry j 1 and trafficking to immune cells in gut lumen.
日本扁柏的主要过敏原 Cry j 1 通过在 65%相对湿度下的干热美拉德反应与半乳甘露聚糖（Mw 为 15 kDa）、葡聚糖（Mw 为 12 kDa）、木葡聚糖（Mw 为 1.4 kDa）和各种单糖进行缀合。Cry j 1-半乳甘露聚糖缀合物完全掩盖了过敏原 Cry j 1 的表位。Cry j 1-葡聚糖缀合物也掩盖了 Cry j 1 的表位。较小的寡糖（木葡聚糖）和各种单糖的大小不能掩盖过敏原 Cry j 1 的表位。这表明附着的糖的较大分子量对于空间位阻掩蔽表位位点很重要。Cry j 1-半乳甘露聚糖和 Cry j 1-甘露糖缀合物有效地在肠道中运输，并与免疫细胞（如肠道中的树突状细胞）共定位，表明 Cry j 1-糖缀合物通过免疫细胞中的甘露糖受体被吞噬。这些结果表明，Cry j 1-半乳甘露聚糖缀合物适合掩盖 Cry j 1 的表位并在肠道腔中的免疫细胞中运输。
