对氯汞苯磺酸盐阻断并逆转了氨氯地平对兔结肠体外钠转运的作用。
P-chloromercuribenzene sulfonate blocks and reverses the effect of amiloride on sodium transport across rabbit colon in vitro.
作者信息
Gottlieb G P, Turnheim K, Frizzell R A, Schultz S G
出版信息
Biophys J. 1978 Apr;22(1):125-9. doi: 10.1016/S0006-3495(78)85477-0.
Abstract
The addition of 10(-3) M p-chloromercuribenzene sulfonate (PCMBS) to the solution bathing the mucosal surface of rabbit colon has no effect on the rate of active Na transport but blocks or reverses the inhibitory action of amiloride. The tissue must be exposed to PCMBS for 20-30 min for a complete blocking effect, and removal of PCMBS from the mucosal solution after this period of exposure does not restore the sensitivity of the tissue to amiloride. The slow time-courses of the blocking and reversal effects suggest that PCMBS does not irreversibly interact with groups directly involved in the binding of amiloride.
摘要
向灌注兔结肠黏膜表面的溶液中添加10⁻³M对氯汞苯磺酸盐（PCMBS）对主动钠转运速率没有影响，但会阻断或逆转阿米洛利的抑制作用。组织必须暴露于PCMBS 20 - 30分钟才能产生完全的阻断作用，在此暴露期后从黏膜溶液中去除PCMBS并不能恢复组织对阿米洛利的敏感性。阻断和逆转作用的缓慢时间进程表明，PCMBS不会与直接参与阿米洛利结合的基团发生不可逆相互作用。
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