Townson S, Tagboto S K
CAB International Institute of Parasitology, St. Albans, Hertfordshire, UK.
Trop Med Parasitol. 1991 Mar;42(1):31-7.
Experiments examined the sensitivity of Onchocerca lienalis microfilariae (mf) to ivermectin in vitro using a range of parameters to measure viability. Incubation in drug (1 x 10(-4) and 5.71 x 10(-8) M) caused an immediate decrease in motility levels in a concentration dependent fashion with a continuing decline over 24 h; the inclusion of a monkey kidney cell (LLCMK2) feeder layer in the culture system partially abrogated drug effects. The use of feeder cells allowed the longer-term culture of mf; incubation in drug at low concentrations (5.71 x 10(-8) and 1 x 10(-10) M) significantly reduced motility levels during a 17 day trial. Using MTT colorimetry it was found that 10,000 untreated mf per replicate sample were required to produce a sufficient quantity of formazan to make comparisons, and that formazan formation was most rapid during the first 30 min of incubation in MTT but continued up to 3-4 h. Following incubation of mf in ivermectin (1 x 10(-4) and 5.71 x 10(-8) M) for 24 h, only the higher concentration inhibited formazan formation (51%), while motility levels were reduced by 94% and 45% respectively. Using the same drug concentrations, mf viability was assessed by measuring the uptake of [3H]2-deoxy-D-glucose; uptake was only significantly reduced (40%) by the higher concentration, while motility levels were reduced by 77% and 63% respectively.(ABSTRACT TRUNCATED AT 250 WORDS)
实验使用一系列测量活力的参数，在体外检测链尾盘尾丝虫微丝蚴（mf）对伊维菌素的敏感性。在药物（1×10⁻⁴和5.71×10⁻⁸M）中孵育导致活力水平立即以浓度依赖性方式下降，并在24小时内持续下降；在培养系统中加入猴肾细胞（LLCMK2）饲养层可部分消除药物作用。使用饲养细胞可实现mf的长期培养；在低浓度（5.71×10⁻⁸和1×10⁻¹⁰M）药物中孵育，在17天的试验期间显著降低了活力水平。使用MTT比色法发现，每个重复样本需要10,000条未处理的mf才能产生足够量的甲臜以进行比较，并且甲臜形成在MTT孵育的前30分钟内最快，但可持续长达3 - 4小时。将mf在伊维菌素（1×10⁻⁴和5.71×10⁻⁸M）中孵育24小时后，只有较高浓度抑制甲臜形成（51%），而活力水平分别降低了94%和45%。使用相同的药物浓度，通过测量[³H]2 - 脱氧 - D - 葡萄糖的摄取来评估mf活力；只有较高浓度使摄取量显著降低（40%），而活力水平分别降低了77%和63%。（摘要截断于250字）
