正常男性和 HIV 感染男性生殖道组织中 Toll 样受体的表达。
Expression of toll-like receptors in genital tract tissues from normal and HIV-infected men.
机构信息
Department of Obstetrics/Gynecology, Boston University School of Medicine, Boston, MA 02118, USA.
出版信息
Am J Reprod Immunol. 2011 Jan;65(1):28-43. doi: 10.1111/j.1600-0897.2010.00877.x.
PROBLEM
cells of the innate immune system use Toll-like receptors (TLRs) to recognize and respond to invading pathogens. This study was carried out to characterize TLR expression in the human male genital tract, an initial infection site for several sexually transmitted pathogens.
METHOD OF STUDY
immunohistochemistry was used to detect expression of TLRs 1-9 in genital tract tissues from HIV(-) and HIV(+) men.
RESULTS
in HIV(-) men, TLR1(+) leukocytes were detected throughout the genital tract. Leukocytes in the penile urethra also expressed TLRs2, 3, 5, 7 and 9. Epithelial cells in most tissues did not express TLRs; exceptions were the prostate, where TLRs3 and 8 were observed on the apical surface of luminal epithelial cells, and the penile urethra, where epithelial cells expressed TLR9. In genital tissues from HIV(+) men with AIDS, few TLR(+) cells were detected.
CONCLUSION
cells in the male genital tract can express a variety of TLRs. The penile urethra contained the highest number of TLR(+) cells, indicating that this tissue plays a major role in the innate immune defense of the male genital tract. Overall, TLR expression was reduced in genital tissues from HIV(+) men.
问题
先天免疫系统的细胞利用 Toll 样受体（TLRs）来识别和响应入侵的病原体。本研究旨在描述 TLR 在人体男性生殖道中的表达，因为该部位是多种性传播病原体的初始感染部位。
研究方法
采用免疫组织化学方法检测 TLR1-9 在 HIV（-）和 HIV（+）男性生殖道组织中的表达。
结果
在 HIV（-）男性中，TLR1（+）白细胞存在于整个生殖道中。阴茎尿道中的白细胞也表达 TLR2、3、5、7 和 9。大多数组织中的上皮细胞不表达 TLRs；但也有例外，如前列腺中，腔上皮细胞的顶表面表达 TLR3 和 8，而阴茎尿道中，上皮细胞表达 TLR9。在 AIDS 合并 HIV（+）男性的生殖道组织中，检测到的 TLR（+）细胞很少。
结论
男性生殖道中的细胞可以表达多种 TLRs。阴茎尿道中含有最多的 TLR（+）细胞，表明该组织在男性生殖道的先天免疫防御中起着重要作用。总的来说，HIV（+）男性的生殖道组织中 TLR 的表达减少。
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