ASL Frosinone, Rome, Italy.
Anticancer Res. 2010 Apr;30(4):1287-92.
HER2 gene amplification was explored using the silver stain hybridization in situ (SISH) technique in colon, prostate, lung, ovarian and breast carcinomas. Clinical pathological features and immunohistochemical (IHC) expression were evaluated for HER2 in 225 carcinomas. All cases were subjected to SISH investigation. Statistical analysis revealed an association between HER2 protein expression and gene amplification in breast carcinoma. 14% of colon carcinomas (5 IHC score 0, 1 score 1+ and 1 score 2+), 2% of prostate carcinoma (IHC 2+), 4% of lung carcinomas (IHC 2+) and 16% ovarian carcinomas (IHC 3+) revealed gene amplification. SISH is an advantageous technique for the detection of gene amplification. The use of the SISH technique in breast carcinoma may be an alternative to other in situ hybridization (ISH) techniques however more detailed studies seem necessary to detect HER2 gene amplification in other human malignancies.
使用银染原位杂交（SISH）技术检测了结肠癌、前列腺癌、肺癌、卵巢癌和乳腺癌中的 HER2 基因扩增。在 225 例癌中评估了 HER2 的临床病理特征和免疫组化（IHC）表达。所有病例均进行 SISH 检测。统计分析显示，乳腺癌中 HER2 蛋白表达与基因扩增之间存在关联。14%的结肠癌（5 个 IHC 评分 0、1 个评分 1+和 1 个评分 2+）、2%的前列腺癌（IHC 2+）、4%的肺癌（IHC 2+）和 16%的卵巢癌（IHC 3+）显示基因扩增。SISH 是一种用于检测基因扩增的优势技术。SISH 技术在乳腺癌中的应用可能是其他原位杂交（ISH）技术的替代方法，但需要进行更详细的研究来检测其他人类恶性肿瘤中的 HER2 基因扩增。
