广西壮族人群中CCR5-Δ32、CCR2-64I和SDF1-3'A的分布情况。
Distribution of CCR5-{delta}32, CCR2-64I, and SDF1-3'A in Guangxi Zhuang population.
机构信息
Center for AIDS Research & School of Public Health, Guangxi Medical University, Nanning, China.
出版信息
J Int Assoc Physicians AIDS Care (Chic). 2010 May-Jun;9(3):145-9. doi: 10.1177/1545109710367517.
BACKGROUND
To investigate the distribution of CCR5-Delta32, CCR2-64I, and SDF1-3'A alleles in Guangxi province Zhuang population.
METHOD
A total of 180 healthy individuals and 150 HIV-positive patients were recruited. All individuals were genotyped for CCR5 polymorphism using polymerase chain reaction (PCR), and CCR2 and SDF1 polymorphism using PCR restriction fragment length polymorphisms (PCR-RFLPs).
RESULTS
No CCR5-Delta32 mutation was found. CCR2-64I frequency was 24.7% in the healthy group and 20.3% in the HIV-positive group, and SDF1-3'A was 30.6% and 29.3% in the 2 groups, respectively. There were no significances in the frequencies of CCR2-64I (chi(2) = 1.795, P = .180) and SDF1-3'A (chi(2) = 0.116, P = .733) between the 2 groups.
CONCLUSIONS
The protective CCR5-Delta32 mutation is absent in the Zhuang population, but CCR2-64I and SDF1-3'A are relatively common and seem not to confer protection against HIV-1 infection. The results suggest that the Zhuang people may have a similar genetic susceptibility to HIV-1 infection with most other Chinese ethnic groups.
背景
研究广西壮族人群中CCR5-Delta32、CCR2-64I和SDF1-3'A等位基因的分布情况。
方法
共招募了180名健康个体和150名HIV阳性患者。所有个体均采用聚合酶链反应（PCR）对CCR5多态性进行基因分型，采用PCR限制性片段长度多态性（PCR-RFLPs）对CCR2和SDF1多态性进行基因分型。
结果
未发现CCR5-Delta32突变。CCR2-64I在健康组中的频率为24.7%，在HIV阳性组中为20.3%；SDF1-3'A在两组中的频率分别为30.6%和29.3%。两组之间CCR2-64I（χ² = 1.795，P = 0.180）和SDF1-3'A（χ² = 0.116，P = 0.733）的频率无显著差异。
结论
壮族人群中不存在具有保护作用的CCR5-Delta32突变，但CCR2-64I和SDF1-3'A相对常见，且似乎不具有抵御HIV-1感染的保护作用。结果表明，壮族人群对HIV-1感染的遗传易感性可能与大多数其他中国民族相似。
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