Mounicou Sandra, Szpunar Joanna, Lobinski Ryszard
CNRS/UPPA UMR5254, Laboratoire de Chimie Analytique Bio-Inorganique et Environnement (LCABIE), Hélioparc, 2, av. Pr. Angot, Pau, France.
Eur J Mass Spectrom (Chichester). 2010;16(3):243-53. doi: 10.1255/ejms.1059.
The potential of inductively-coupled plasma mass spectrometry (ICP-MS) and its complementarity to soft- ionization MS techniques are discussed in the context of the analysis for biomolecules. ICP-MS offers detection limits in the attomolar range, regardless of the molecular environment of the target element. The sensitivity is hardly affected by the sample matrix, chromatographic mobile phase, or co-eluted compounds. The abundance sensitivity over six decades and the linear dynamic range over nine decades make simultaneous multi-isotopic analysis routinely possible. The manuscript discusses the state-of-the-art of ICP-MS for the detection of proteins in gel electrophoresis and of peptides in 2D high-performance liquid chromatography. The possibilities of quantification to the degree of some post-translational modifications are highlighted. Attention is also paid to the role of ICP-MS in protein quantification via metal-coded labeling and to the use of differentially-labeled antibodies for the multiplexed biomarker analysis. The key role of ICP-MS in the emerging area of metallomics is briefly discussed.
在生物分子分析的背景下，讨论了电感耦合等离子体质谱（ICP-MS）的潜力及其与软电离质谱技术的互补性。ICP-MS提供了阿托摩尔范围内的检测限，而与目标元素的分子环境无关。灵敏度几乎不受样品基质、色谱流动相或共洗脱化合物的影响。超过六个数量级的丰度灵敏度和超过九个数量级的线性动态范围使同时进行多同位素分析成为常规可能。该手稿讨论了用于检测凝胶电泳中蛋白质和二维高效液相色谱中肽的ICP-MS的最新技术。强调了对某些翻译后修饰程度进行定量的可能性。还关注了ICP-MS在通过金属编码标记进行蛋白质定量中的作用以及使用差异标记抗体进行多重生物标志物分析。简要讨论了ICP-MS在新兴的金属组学领域中的关键作用。
