用聚乙二醇修饰氨基对来自苛求芽孢杆菌的重组尿酸酶的影响。
Effects of modification of amino groups with poly(ethylene glycol) on a recombinant uricase from Bacillus fastidiosus.
作者信息
Zhang Chun, Yang Xiaolan, Feng Juan, Yuan Yonghua, Li Xiang, Bu Youquan, Xie Yanling, Yuan Huidong, Liao Fei
机构信息
Unit for Biotransformation and Protein Biotechnology, Chongqing Key Laboratory of Biochemical and Molecular Pharmacology, Chongqing Medical University, Chongqing, China.
出版信息
Biosci Biotechnol Biochem. 2010;74(6):1298-301. doi: 10.1271/bbb.100080. Epub 2010 Jun 7.
After modification with monomethoxyl-poly(ethylene glycol)-5000, a recombinant intracellular uricase from Bacillus fastidiosus ATCC 29604 showed residual activity of about 65%, a thermo-inactivation half-life >85 h, a circulating half-life about 20 h in rats in vivo, consistent effects of common cations, and consistent optima for reaction temperature and pH. Thus, this uricase can be formulated via modification with monomethoxyl-poly(ethylene glycol).
经甲氧基聚乙二醇-5000修饰后，来自苛求芽孢杆菌ATCC 29604的重组细胞内尿酸酶显示出约65%的残余活性、热失活半衰期>85小时、在大鼠体内的循环半衰期约20小时、常见阳离子的作用一致以及反应温度和pH的最佳值一致。因此，这种尿酸酶可以通过甲氧基聚乙二醇修饰来进行制剂化。
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