肾移植中环孢素监测的非特异性放射免疫测定、高效液相色谱法和荧光偏振免疫测定的比较。

Comparison of nonspecific radioimmunoassay, high-performance liquid chromatography, and fluorescence polarization immunoassay for cyclosporine monitoring in renal transplantation.

作者信息

Lee S F, Yang W C, Shann T Y, Lui W Y, Wang R B

机构信息

Division of Clinical Pharmacy, Veterans General Hospital-Taipei, Taiwan, Republic of China.

出版信息

Ther Drug Monit. 1991 Mar;13(2):152-6. doi: 10.1097/00007691-199103000-00011.

DOI:10.1097/00007691-199103000-00011

PMID:2053123

Abstract

Three methods, i.e., nonspecific radioimmunoassay (RIA; Incstar), fluorescence polarization immunoassay (FPIA; TDx Abbott), and high-performance liquid chromatography (HPLC), have been used for monitoring cyclosporine blood levels in renal transplantation patients. The levels obtained from 135 samples showed a modest correlation between RIA and HPLC, FPIA and HPLC, RIA and FRIA. The mean ratios of RIA to HPLC, FPIA to HPLC, and RIA to FPIA were 2.96, 4.14, and 0.73. The significant variations in cyclosporine levels result from the cross-reaction of antibody with some cyclosporine metabolites, by which these two methods often overestimate the true blood cyclosporine level. HPLC is a more effective and reliable method for pharmacokinetic studies and blood level monitoring of cyclosporine in clinical practice.

摘要

三种方法，即非特异性放射免疫测定法（RIA；Incstar公司）、荧光偏振免疫测定法（FPIA；雅培TDx）和高效液相色谱法（HPLC），已用于监测肾移植患者的环孢素血药浓度。从135个样本中获得的浓度显示，RIA与HPLC、FPIA与HPLC、RIA与FPIA之间存在适度相关性。RIA与HPLC、FPIA与HPLC、RIA与FPIA的平均比值分别为2.96、4.14和0.73。环孢素浓度的显著差异是由于抗体与一些环孢素代谢物的交叉反应导致的，这两种方法常常高估了环孢素的真实血药浓度。在临床实践中，HPLC是一种用于环孢素药代动力学研究和血药浓度监测的更有效、更可靠的方法。