Vandenbroucke A C
Department of Clinical Biochemistry, St. Michael's Hospital, University of Toronto, Ontario, Canada.
Clin Biochem. 1988 Oct;21(5):307-9. doi: 10.1016/s0009-9120(88)80086-9.
The recent introduction of a fluorescence polarization immunoassay (FPIA) for cyclosporine by Abbott for their TDx analyzer provides an alternative to radioimmunoassay (RIA) and high performance liquid chromatography procedures. This new method is easier and faster than the Incstar CYCLO-Trac RIA assay. The TDx method has good precision except at low levels of cyclosporine. RIA gives consistently higher values than FPIA for samples containing only cyclosporine and in those from renal transplant patients. The FPIA procedure may be modified to allow up to a 50-min pause during the protein precipitation step, allowing more efficient use of technologists' time.
雅培公司为其TDx分析仪最近推出的用于环孢素的荧光偏振免疫测定法（FPIA），为放射免疫测定法（RIA）和高效液相色谱法提供了一种替代方法。这种新方法比Incstar CYCLO-Trac RIA测定法更简便、快速。除了在低环孢素水平时，TDx方法具有良好的精密度。对于仅含环孢素的样品以及肾移植患者的样品，RIA给出的值始终高于FPIA。FPIA程序可以修改，以便在蛋白质沉淀步骤中允许长达50分钟的暂停，从而更有效地利用技术人员的时间。
