Kim S U, Pleasure D E
Brain Res. 1978 Apr 21;145(1):15-25. doi: 10.1016/0006-8993(78)90792-8.
Explants from newborn mouse cerebellum were cultured in nutrient media containing either adequate (30%) or low (15% or 7.5%) serum content. By light microscopic observation, delayed and inhibited myelination was detected in cultures fed with low serum media (experimental cultures). Specific activities of two enzymes related to myelin synthesis, 2',3'-cyclic nucleotide-3'-phosphohydrolase and 3-hydroxy-3-methylglutaryl coenzyme A reductase, were also reduced in experimental cultures. Morphometric analysis of electron micrographs showed that the size of presynaptic endings and total area occupied by synapses in experimental cultures were substantially reduced, while synaptic density per unit area increased. Reflecting the results of synaptic underdevelopment, the levels of two neurotransmitter enzymes, choline acetyltransferase and glutamic acid decarboxylase, were also decreased in experimental cultures.
将新生小鼠小脑的外植体培养在含有充足（30%）或低（15%或7.5%）血清含量的营养培养基中。通过光学显微镜观察，在低血清培养基喂养的培养物（实验性培养物）中检测到髓鞘形成延迟和受抑制。与髓鞘合成相关的两种酶，即2',3'-环核苷酸-3'-磷酸水解酶和3-羟基-3-甲基戊二酰辅酶A还原酶的比活性在实验性培养物中也降低。电子显微镜照片的形态计量分析表明，实验性培养物中突触前末梢的大小和突触占据的总面积大幅减少，而单位面积的突触密度增加。反映突触发育不全的结果，实验性培养物中两种神经递质酶，即胆碱乙酰转移酶和谷氨酸脱羧酶的水平也降低。
