Takaba H, Nagai T, Hashimoto J, Yamamoto M, Miyake K
Department of Urology, Nagoya University School of Medicine, Japan.
Urol Int. 1991;46(2):180-3. doi: 10.1159/000282128.
SDS-PAGE of pepsin-solubilized collagens obtained from testes of elderly men revealed types I, III, V, and VI but not type IV. The total collagen content was 21.3 +/- 1.96% (mean +/- SD) as determined by hydroxyproline assay. The ratio for the pepsin-soluble fraction was 7.98 +/- 3.27%, i.e. very low as compared with other tissues. Type I collagen was determined by SDS-PAGE of cyanogen bromide cleavage of the pepsin-insoluble residue.
对从老年男性睾丸中提取的胃蛋白酶可溶解胶原蛋白进行的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）显示存在I型、III型、V型和VI型胶原蛋白，但不存在IV型。通过羟脯氨酸测定法确定，总胶原蛋白含量为21.3±1.96%（平均值±标准差）。胃蛋白酶可溶部分的比例为7.98±3.27%，即与其他组织相比非常低。通过对胃蛋白酶不溶性残渣的溴化氰裂解产物进行SDS-PAGE测定I型胶原蛋白。
