Takaba H, Nagai T, Hashimoto J, Yamamoto M, Miyake K
Department of Urology, Nagoya University School of Medicine, Japan.
Urol Int. 1991;46(2):180-3. doi: 10.1159/000282128.
SDS-PAGE of pepsin-solubilized collagens obtained from testes of elderly men revealed types I, III, V, and VI but not type IV. The total collagen content was 21.3 +/- 1.96% (mean +/- SD) as determined by hydroxyproline assay. The ratio for the pepsin-soluble fraction was 7.98 +/- 3.27%, i.e. very low as compared with other tissues. Type I collagen was determined by SDS-PAGE of cyanogen bromide cleavage of the pepsin-insoluble residue.