McVey D S, Shuman W
Department of Laboratory Medicine, College of Veterinary Medicine, Kansas State Veterinary Medical Center, Manhattan.
Vet Immunol Immunopathol. 1991 Mar;28(1):37-43. doi: 10.1016/0165-2427(91)90041-a.
The presence of antinuclear antibody in serum has been used to serologically support the clinical diagnosis of systemic lupus erythematosus. Most often, diagnostic support is conferred if the titer is above a "cut-off" value determined by the particular laboratory. The precision estimates of these commonly used serological assays are high. The arithmetic and geometric precisions estimates of fluorescent antinuclear antibody (FANA) assays were determined utilizing sera from dogs with polysystemic signalment suggestive of SLE. A simple score assay was found to have improved precision over FANA endpoint titrations on Hep-2 or cryostat, rat liver substrates. In one case, the precision improved from an arithmetic coefficient of variation of 25.6% (HEp-2, endpoint dilution, FANA titers) or 19.7% (rat liver cryostat substrate FANA titers) to 15.5%. Geometric coefficients of variation followed similar trends. Further, the score values for dogs with SLE were considerably higher (mean = 3.28) than for clinically normal dogs (mean = 0.20). The score system was more precise than FANA endpoint titers and therefore may be useful for diagnostic purposes, especially when FANA titers are negative or low.
血清中抗核抗体的存在已被用于从血清学上支持系统性红斑狼疮的临床诊断。大多数情况下，如果滴度高于特定实验室确定的“临界值”，则可提供诊断支持。这些常用血清学检测方法的精密度估计值较高。利用有多系统症状提示系统性红斑狼疮的犬只血清，确定了荧光抗核抗体（FANA）检测的算术和几何精密度估计值。发现一种简单的评分检测方法比在人喉癌上皮细胞（HEp-2）或低温恒温器处理的大鼠肝脏底物上进行的FANA终点滴定法具有更高的精密度。在一个案例中，精密度从算术变异系数25.6%（HEp-2，终点稀释，FANA滴度）或19.7%（大鼠肝脏低温恒温器底物FANA滴度）提高到了15.5%。几何变异系数也呈现类似趋势。此外，患有系统性红斑狼疮的犬只的评分值（平均值 = 3.28）明显高于临床正常犬只（平均值 = 0.20）。该评分系统比FANA终点滴度更精确，因此可能对诊断有帮助，特别是当FANA滴度为阴性或较低时。
