Genome Research Chair, Biochemistry Department, Faculty of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia.
Protein J. 2010 Jul;29(5):306-13. doi: 10.1007/s10930-010-9253-5.
The expression levels of cytochrome P450s were examined in different camel tissues by western blotting and semi-quantitative polymerase chain reaction. Camelus dromedarius liver microsomes were found to express different P450s isoenzymes constitutively. The maximum expression of P450 protein was seen in the camel liver in the order of P450 2E1, 1A1, 3A and 2B1/2. Camel extrahepatic tissues, kidney, spleen and the lung showed detectable levels of P450s 1A1 but lower than that noticed in liver. Detectable level of P450 2B1/2 was also observed in camel lung (29.5 vs. 58% liver microsomes). P450scc and 21-hydroxylase were found to be differentially expressed only in camel testis. Partial sequences of these P450s genes showed high similarities with the human P450s. These results demonstrate that the multiple forms of P450s are differentially expressed in camel tissues and that the relative levels of expression are comparable with other mammals. These observations might be important in understanding the differential susceptibility of camel tissues to the toxic effects of xenobiotics and environmental pollution.
通过 Western blot 和半定量聚合酶链反应检测了不同骆驼组织中细胞色素 P450 的表达水平。发现骆驼肝微粒体中组成性表达不同的 P450 同工酶。P450 蛋白的最大表达在骆驼肝脏中按 P450 2E1、1A1、3A 和 2B1/2 的顺序出现。骆驼肝外组织如肾、脾和肺中可检测到 P450s 1A1，但低于肝脏中检测到的水平。在骆驼肺中也可检测到 P450 2B1/2（29.5%对 58%的肝微粒体）。仅在骆驼睾丸中发现 P450scc 和 21-羟化酶的差异表达。这些 P450 基因的部分序列与人 P450s 高度相似。这些结果表明，多种形式的 P450 在骆驼组织中差异表达，表达水平与其他哺乳动物相当。这些观察结果可能对理解骆驼组织对异生物质和环境污染的毒性作用的不同敏感性具有重要意义。
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