Biochemistry Department, College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia.
Biochem Genet. 2012 Apr;50(3-4):285-97. doi: 10.1007/s10528-011-9471-3. Epub 2011 Oct 1.
This study determined the full-length sequence of CYP2E1, one of six cytochrome P450 genes previously examined in camel tissues by western blotting and semi-quantitative PCR. The Camelus dromedarius CYP2E1 has an open reading frame of 1,473 bp, and the cDNA encodes a protein of 490 amino acid residues with a molecular weight of 54.8 kDa. The deduced amino acid sequence showed the highest identity with Bos taurus (88%), Sus scrofa (87%), and Homo sapiens (83%). In a phylogenetic analysis, the C. dromedarius CYP2E1 isoform was located beside cattle and pigs. The deduced amino acid sequence of camel CYP2E1 showed the conserved proline-rich amino terminus and the heme-binding signature localized near the carboxy terminus of the protein.
本研究确定了 CYP2E1 的全长序列，CYP2E1 是之前通过 Western blot 和半定量 PCR 检测到的骆驼组织中的六个细胞色素 P450 基因之一。Camelus dromedarius CYP2E1 的开放阅读框为 1473bp，cDNA 编码一个 490 个氨基酸残基的蛋白质，分子量为 54.8kDa。推导的氨基酸序列与 Bos taurus（88%）、Sus scrofa（87%）和 Homo sapiens（83%）具有最高的同一性。在系统发育分析中，C. dromedarius CYP2E1 同工型位于牛和猪旁边。骆驼 CYP2E1 的推导氨基酸序列显示出保守的富含脯氨酸的氨基末端和位于蛋白质羧基末端附近的血红素结合特征。
