Qian Zhen, Lin Jiang, Qian Jun, Yao Dong-ming, Wang Ya-li, Han Lan-xiu, Zhu Zhao-hui, Xiao Gao-fei
Department of Hematology, Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu, 212002 PR China.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2010 Jun;27(3):290-3. doi: 10.3760/cma.j.issn.1003-9406.2010.0.012.
To quantify the expression level of GRAF gene in acute myeloid leukemia (AML) and analyze its clinical significance.
The EvaGreen real-time quantitative polymerase chain reaction (RQ-PCR) assay was established and performed to measure the GRAF gene transcripts in 71 cases with AML and 21 with nonmalignant hematological diseases. The clinical correlation of GRAF expression was analyzed.
The established EvaGreen RQ-PCR assay had good specificity, reproducibility and sensitivity. The GRAF expression level was significantly lower in AML (0.01%-169.75%, median 3.82%) than that in controls (14.49%-126.85%, median 56.04%) (P<0.05). There was no correlation between the level of GRAF transcript and the sex, age, hematologic parameters, FAB subtypes and karyotypic groups (P>0.05).
The GRAF gene was down-regulated in AML, which might play a role in the leukemogenesis.
量化GRAF基因在急性髓系白血病（AML）中的表达水平，并分析其临床意义。
建立并进行EvaGreen实时定量聚合酶链反应（RQ-PCR）检测，以测定71例AML患者和21例非恶性血液病患者的GRAF基因转录本。分析GRAF表达的临床相关性。
建立的EvaGreen RQ-PCR检测具有良好的特异性、重复性和敏感性。AML中GRAF表达水平（0.01%-169.75%，中位数3.82%）显著低于对照组（14.49%-126.85%，中位数56.04%）（P<0.05）。GRAF转录本水平与性别、年龄、血液学参数、FAB亚型和核型分组之间无相关性（P>0.05）。
AML中GRAF基因下调，可能在白血病发生中起作用。
