Ministry of Education Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, P. R. China.
J Sep Sci. 2010 Jul;33(14):2145-52. doi: 10.1002/jssc.201000074.
A sensitive, fast and simple method based on magnetic octadecylsilane particles was developed for the extraction of three fat-soluble vitamins followed by capillary LC (CLC) analysis with UV detection. Magnetic octadecylsilane particles were prepared based on three-step reactions including co-precipitation, sol-gel polymerization and alkylation. The characterization of the prepared product was studied by scanning electron microscope and Fourier-transform infrared spectrometry. The particles were used as magnetic SPE adsorbent for the extraction of fat-soluble vitamins in fruit juice-milk beverage. The extraction condition and efficiency of the particles for fat-soluble vitamins were investigated. By coupling magnetic SPE with capillary LC with UV detection, low concentrations of fat-soluble vitamins in fruit juice-milk beverage can be detected without the interference from other substances in the sample matrix.
建立了一种基于十八烷基硅烷磁性粒子的灵敏、快速、简单的方法，用于提取三种脂溶性维生素，然后采用毛细管 LC（CLC）进行分析，并用紫外检测。基于共沉淀、溶胶-凝胶聚合和烷基化三步反应制备了十八烷基硅烷磁性粒子。通过扫描电子显微镜和傅里叶变换红外光谱研究了制备产物的特性。将粒子用作果汁-牛奶饮料中脂溶性维生素的磁性 SPE 吸附剂。考察了粒子对脂溶性维生素的萃取条件和效率。通过将磁性 SPE 与毛细管 LC 与紫外检测相结合，可以在不干扰样品基质中其他物质的情况下，检测果汁-牛奶饮料中低浓度的脂溶性维生素。
