School of Chemistry, Cardiff University, Main Building, Park Place, Cardiff CF10 3AT, UK.
Macromol Biosci. 2010 Aug 11;10(8):963-73. doi: 10.1002/mabi.201000040.
The mechanism of ISA23 · HCl interaction with model membrane vesicles (80-100 nm in diameter) was investigated using EPR in conjunction with SANS. For EPR, 16-DSE was dissolved in the vesicle membrane to measure its dynamics and polarity, whereas a spin-labeled (Tempo)-ISA 23 analogue was used to give a measure of the polymer flexibility. When ISA23 was added to the external vesicle surface, no interaction was found. This observation conflicts with the reported ability to lyse RBC, but is in agreement with recent studies that showed no effect on membrane permeability when a PAA was added to an incubation medium containing isolated lysosomal vesicles. The vesicle-mimetic models used here provide a new and useful tool for studying endosomolytic polymer/membrane interactions.
采用 EPR 与 SANS 相结合的方法研究了 ISA23·HCl 与模型膜泡（直径 80-100nm）的相互作用机制。对于 EPR，将 16-DSE 溶解在囊泡膜中以测量其动力学和极性，而使用标记有自旋的（Tempo）-ISA 23 类似物来测量聚合物的柔韧性。当 ISA23 添加到外部囊泡表面时，未发现相互作用。这一观察结果与报道的裂解 RBC 的能力相矛盾，但与最近的研究结果一致，即在含有分离的溶酶体囊泡的孵育介质中添加 PAA 时，对膜通透性没有影响。这里使用的囊泡模拟模型为研究内溶酶体聚合物/膜相互作用提供了一种新的有用工具。
