Department of Conservative Dentistry, The Institute of Oral Health Science, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
Oper Dent. 2010 May-Jun;35(3):330-6. doi: 10.2341/09-244-L.
This study evaluated the effects of blood contamination and decontamination methods during different steps of bonding procedures on the microtensile bond strength of two-step self-etch adhesives to dentin. Sixty extracted human molars were ground flat to expose occlusal dentin. The 60 molars were randomly assigned to three groups, each treated with a different two-step self-etch adhesive: Clearfil SE Bond, AdheSE and Tyrian SPE. In turn, these groups were subdivided into five subgroups (n = 20), each treated using different experimental conditions as follows: control group-no contamination; contamination group 1-CG1: primer application/ contamination/primer re-application; contamination group 2-CG2: primer application/contamination/wash/dry/primer re-application; contamination group 3-CG3: primer application/adhesive application/light curing/contamination/ adhesive re-application/light curing; contamina- tion group 4-CG4: primer application/adhesive application/light curing/contamination/wash/ dry/adhesive re-application/light curing. Composite buildup was performed using Z250. After 24 hours of storage in distilled water at 37 degrees C, the bonded specimens were trimmed to an hourglass shape and serially sectioned into slabs with 0.6 mm2 cross-sectional areas. Microtensile bond strengths (MTBS) were assessed for each specimen using a universal testing machine. The data were analyzed by two-way ANOVA followed by a post hoc LSD test. SEM evaluations of the fracture modes were also performed. The contaminated specimens showed lower bond strengths than specimens in the control group (p < 0.05), with the exception of CG1 in the Clearfil SE group and CG2 and CG3 in the Tyrian SPE group. Among the three self-etch adhesives, the Tyrian SPE group exhibited a significantly lower average MTBS compared to the Clearfil SE Bond and AdheSE (p < 0.05) groups. Based on the results of the current study, it was found that blood contamination reduced the MTBS of all three self-etch adhesives to dentin, and water-rinsing was unable to overcome the effects of blood contamination.
本研究评估了在粘结程序的不同步骤中血液污染和去污方法对两步自酸蚀粘结剂与牙本质之间微拉伸粘结强度的影响。从 60 个人工磨牙中取出，磨平以暴露咬合面牙本质。将这 60 个磨牙随机分为三组，每组用不同的两步自酸蚀粘结剂处理：Clearfil SE Bond、AdheSE 和 Tyrian SPE。然后，这些组又细分为五个亚组（n = 20），每个亚组用不同的实验条件处理如下：对照组-无污染；污染组 1-CG1：底漆应用/污染/底漆再应用；污染组 2-CG2：底漆应用/污染/水洗/干燥/底漆再应用；污染组 3-CG3：底漆应用/粘结剂应用/光固化/污染/粘结剂再应用/光固化；污染组 4-CG4：底漆应用/粘结剂应用/光固化/污染/水洗/干燥/粘结剂再应用/光固化。使用 Z250 进行复合堆积。在 37 摄氏度的蒸馏水中储存 24 小时后，将粘结的样本修剪成沙漏形状，并将其连续切成具有 0.6mm2 横截面的薄片。使用万能试验机对每个样本进行微拉伸粘结强度（MTBS）评估。使用双因素方差分析（ANOVA）和事后 LSD 检验对数据进行分析。还对断裂模式进行了扫描电镜（SEM）评估。污染样本的粘结强度低于对照组样本（p < 0.05），但 Clearfil SE 组的 CG1 和 Tyrian SPE 组的 CG2 和 CG3 除外。在三种自酸蚀粘结剂中，Tyrian SPE 组的平均 MTBS 明显低于 Clearfil SE Bond 和 AdheSE 组（p < 0.05）。根据本研究的结果，发现血液污染降低了所有三种自酸蚀粘结剂与牙本质之间的 MTBS，水冲洗无法克服血液污染的影响。
