AB Sciex, via Tiepolo 18, 20052 Monza, Italy.
Anal Chem. 2010 Jul 1;82(13):5636-45. doi: 10.1021/ac100607v.
The study deals with a fast (analysis times of around 11 min) simultaneous identification and quantification in human urine of 42 drugs (21 therapeutic and 21 of abuse), through an automated online solid phase extraction ultra high performance liquid chromatography method coupled with tandem mass spectrometry (SPE UHPLC-MS/MS). In the method validation, particular attention was devoted to the matrix effect, through matrix-matched calibration in blank urine, suitably diluted. For all the abuse drugs investigated, the limit of quantitation (LOQ) values are lower than the legal threshold concentration levels, making the method suitable for routine control. The whole procedure was applied in the analysis of urine of patients positive to the I level screening test.
本研究建立了一种快速（分析时间约 11 分钟）同时鉴定和定量检测人尿液中 42 种药物（21 种治疗药物和 21 种滥用药物）的方法，采用自动化在线固相萃取超高效液相色谱-串联质谱法（SPE-UHPLC-MS/MS）。在方法验证中，特别关注基质效应，通过适当稀释空白尿液进行基质匹配校准。对于所有研究的滥用药物，定量限（LOQ）值均低于法定阈值浓度水平，使该方法适用于常规控制。整个过程应用于 I 级筛选试验阳性患者尿液的分析。
