Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
Anal Chem. 2010 Jul 1;82(13):5906-9. doi: 10.1021/ac100941z.
This technical note reports a design utilizing an aerodynamic lens assembly to transport suspended sample particles directly onto the target plate and conduct in situ MALDI analysis. The design provides a new alternative method for online MALDI analysis. The matrix/analyte/salt-contained particles sampled by a nozzle are focused into a particle beam with an aerodynamic lens assembly and then deposited on the target plate directly. The deposited particles are in situ desorbed/ionized by a 266 nm laser beam with an incidence angle of 50 degrees. The generated ions are detected with a reflectron mass spectrometer capable of delayed ion extraction and ion gating. The MALDI-TOF mass spectra of poly(ethylene glycol) (PEG) 1000, 2000, and 4000 particles are obtained in the experiment. The initial experimental results demonstrate that the design can be used for online MALDI analysis.
本技术说明报告了一种设计，利用气动透镜组件将悬浮的样品颗粒直接输送到靶板上，并进行原位 MALDI 分析。该设计为在线 MALDI 分析提供了一种新的替代方法。通过喷嘴采集的基质/分析物/盐包含颗粒被气动透镜组件聚焦成颗粒束，然后直接沉积在靶板上。沉积的颗粒通过入射角为 50 度的 266nm 激光束原位解吸/电离。生成的离子由具有延迟离子提取和离子门控功能的反射质谱仪检测。在实验中获得了聚乙二醇（PEG）1000、2000 和 4000 颗粒的 MALDI-TOF 质谱。初步实验结果表明，该设计可用于在线 MALDI 分析。
