Instituto de Investigaciones Fisicoquímica Teóricas y Aplicadas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, CC 16 Suc. 4, 1900 La Plata, Argentina.
J Biomater Sci Polym Ed. 2010;21(10):1297-312. doi: 10.1163/092050609X12517190417632. Epub 2010 Jun 8.
There is considerable interest in the design of polymeric biomaterials that can be used for the repair of bone defects. In this study, we used ultrasound to prepare a compatibilized blend of poly(epsilon-caprolactone) (PCL) and poly(diisopropyl fumarate) (PDIPF). The formation of post-sonication inter-polymer coupling products was verified by SEC analysis of a blend with azo-labeled PDIPF. We also analyzed the physicochemical and mechanical properties of the compatibilized blend. When compared to PCL alone, the PCL/PDIPF blend showed no difference in its resistance as evaluated by the elastic modulus, although it did show a 50% decrease in ultimate tensile stress (P < 0.05) and an 84% decrease in elongation-at-break (P < 0.05). However, the mechanical properties of this blend were comparable to those of trabecular bone. We next evaluated biocompatibility of the PCL/PDIPF blend, and of homo-polymeric PCL and PDIPF films for comparison, with UMR106 and MC3T3E1 osteoblastic cells. Osteoblasts plated on the compatibilized blend adhered and proliferated more than on either homo-polymer, showed a greater number of cellular processes with a better organized actin cytoskeleton and expressed more type-I collagen and mineral, both markers of osteoblast phenotype. These results support the hypothesis that this new compatibilized blend could be useful in future applications for bone regeneration.
人们对可用于修复骨缺损的聚合物生物材料的设计有着浓厚的兴趣。在这项研究中，我们使用超声制备了聚己内酯（PCL）和聚（二异丙基亚甲基富马酸酯）（PDIPF）的相容共混物。通过对用偶氮标记的 PDIPF 进行 SEC 分析，验证了超声处理后形成的聚合物间偶联产物。我们还分析了相容共混物的物理化学和机械性能。与单独的 PCL 相比，PCL/PDIPF 共混物的弹性模量评价的抗张强度没有差异，尽管其极限拉伸强度降低了 50%（P < 0.05），断裂伸长率降低了 84%（P < 0.05）。然而，该共混物的力学性能与小梁骨相当。接下来，我们评估了 PCL/PDIPF 共混物以及与之相比的均聚物 PCL 和 PDIPF 薄膜的生物相容性，用 UMR106 和 MC3T3E1 成骨细胞进行了评估。与均聚物相比，贴壁和增殖更多的是在相容共混物上的成骨细胞，细胞突起数量更多，肌动蛋白细胞骨架更有组织，表达更多的 I 型胶原和矿物质，这两种标志物都表明成骨细胞表型。这些结果支持了这样一种假设，即这种新的相容共混物可用于未来的骨再生应用。
