Department of Laboratory Medicine, Konventhospital Barmherzige Brueder, Seilerstaette 2-4, A-4020 Linz, Austria.
Clin Biochem. 2010 Sep;43(13-14):1169-70. doi: 10.1016/j.clinbiochem.2010.05.019. Epub 2010 Jun 8.
The aim of this study was to investigate the long-term in vitro stability of soluble ST2 (sST2).
EDTA plasma samples were drawn from 15 individuals with various diseases. The Presage ST2 assay was used for measurement of sST2 concentrations directly after blood collection and after storing plasma samples for 18 months at -20 degrees C and -80 degrees C. The default criterion for analyte stability was set at 95%.
sST2 concentrations in the 15 individuals ranged from 12 U/mL to 140 U/mL. Directly after blood collection, the mean (+/-SD) sST2 concentration was 51+/-37 U/mL, and absolute analyte recoveries were 50+/-35 U/mL and 51+/-34 U/mL after storage of samples for 18 months at -20 degrees C and -80 degrees C, respectively. Relative analyte recoveries after 18 months of storage at -20 degrees C and -80 degrees C were 99+/-5% and 101+/-7%.
sST2 is stable for at least 1.5 years in plasma samples stored at -20 degrees C and -80 degrees C.
本研究旨在探究可溶性 ST2（sST2）的长期体外稳定性。
从 15 名患有各种疾病的个体中抽取 EDTA 血浆样本。采用 Presage ST2 测定法直接测量采血后及在-20°C 和-80°C 下储存血浆样本 18 个月后的 sST2 浓度。分析物稳定性的默认标准设定为 95%。
15 名个体的 sST2 浓度范围为 12 U/mL 至 140 U/mL。直接采血后，sST2 浓度的平均值（+/-SD）为 51+/-37 U/mL，在-20°C 和-80°C 下储存样本 18 个月后，绝对分析物回收率分别为 50+/-35 U/mL 和 51+/-34 U/mL。在-20°C 和-80°C 下储存 18 个月后，相对分析物回收率分别为 99+/-5%和 101+/-7%。
sST2 在-20°C 和-80°C 下储存的血浆样本中至少稳定 1.5 年。
