Magee W E, Cronenberger J H, Thor D E
Cancer Res. 1978 Apr;38(4):1173-6.
A marked stimulation of normal guinea pig lymphocytes was obtained by incubating them with liposomes that contained both antibody to lymphocytes to provide "homing" of the vesicles and immune RNA isolated from guinea pigs immunized with syngeneic line 10 hepatocarcinoma cells. Tumor cell cytotoxicity was monitored by a 51Cr release assay. This target cell delivery of immune RNA by liposomes produced a dose-dependent stimulation up to 12 times that achieved by in vitro methods with naked immune RNA.
通过将正常豚鼠淋巴细胞与脂质体一起孵育，获得了显著的刺激效果。这些脂质体既含有针对淋巴细胞的抗体以促使囊泡“归巢”，又含有从用同基因10型肝癌细胞免疫的豚鼠中分离出的免疫RNA。通过51Cr释放试验监测肿瘤细胞的细胞毒性。脂质体介导的免疫RNA向靶细胞的递送产生了剂量依赖性刺激，其刺激程度比用裸露的免疫RNA进行体外方法所达到的刺激程度高12倍。
