Watanabe H, Suzuki S, Kobayashi M, Usa M, Inaba H
Biophoton Project, Research Development Corporation of Japan, Sendai.
J Biolumin Chemilumin. 1991 Jan-Mar;6(1):13-8. doi: 10.1002/bio.1170060105.
Light emission from Photobacterium phosphoreum was analysed during cell growth on an agar plate from a single cell to colony formation. Temporal analysis of image intensified light was set so that a quadratic window covered a single cell. Intensity of light emission from a single cell through colony formation showed an initial decrease, a prolonged lag phase, and then a rapid increase. These responses on an agar plate were similar to those from liquid cultures. The image analysis showed repeated bursts of light emission in the phases when light was increasing and decreasing. Statistical analysis of light emission also emphasized the presence of bursts of light emission, suggesting the metabolic synchronism of luciferase reactions in either a single cell or suggesting the metabolic synchronism of luciferase reactions in either a single cell or synchronously divided cells. The repetitive bursts of light occurred in a single cell and continued during the growth phase in which the cell population and the light emission was increasing. In a single cell, however, periodicity of light emission was not defined directly from fast Fourier transformation, although it was indicated on oscillation of mean level of fluctuated light emission, at initial phase of culture on agar plate.
在琼脂平板上，从单个细胞到菌落形成的细胞生长过程中，对费氏弧菌的发光情况进行了分析。对图像增强光进行时间分析，设置一个二次窗口覆盖单个细胞。从单个细胞到菌落形成过程中，发光强度呈现出先下降、接着是较长的延迟期、然后快速增加的趋势。在琼脂平板上的这些反应与液体培养中的反应相似。图像分析显示，在光增加和减少的阶段会反复出现发光爆发。对发光的统计分析也强调了发光爆发的存在，这表明在单个细胞中荧光素酶反应存在代谢同步性，或者表明在同步分裂的细胞中荧光素酶反应存在代谢同步性。重复的发光爆发发生在单个细胞中，并在细胞群体和发光增加的生长阶段持续存在。然而，在单个细胞中，虽然在琼脂平板培养的初始阶段，波动发光的平均水平振荡表明了发光的周期性，但无法直接通过快速傅里叶变换来确定发光的周期性。
