Departments of Medicine, Indiana University, Indianapolis, USA.
Am J Physiol Renal Physiol. 2010 Aug;299(2):F453-64. doi: 10.1152/ajprenal.00050.2010. Epub 2010 Jun 9.
Serum amyloid A protein (SAA), a prominent component of the acute-phase response, is strongly expressed in developing and repairing kidneys and promotes tubulogenesis. Accordingly, we reprogrammed relatively undifferentiated NRK52E cells with the mouse SAA1.1 gene and transplanted SAA-positive and -negative cells into rats with acute renal failure. We found that SAA-positive cells accelerated renal recovery in three models of acute renal failure: gentamicin nephrotoxicity, cisplatin-mediated renal injury, and ischemia-reperfusion renal injury. The dramatic improvement of renal failure was demonstrable within 2 days, consistent with an early paracrine effect. However, abundant donor cells were also found integrated in the healing tubular architecture after 7 days. We conclude that infusions of SAA-positive cells promote renal recovery after acute renal failure and offer a potentially powerful and novel therapy of renal failure.
血清淀粉样蛋白 A 蛋白（SAA）是急性期反应的主要成分，在发育和修复肾脏中表达强烈，并促进肾小管发生。因此，我们使用鼠 SAA1.1 基因对相对未分化的 NRK52E 细胞进行重编程，并将 SAA 阳性和阴性细胞移植到急性肾衰竭大鼠体内。我们发现 SAA 阳性细胞在三种急性肾衰竭模型中加速了肾脏恢复：庆大霉素肾毒性、顺铂介导的肾损伤和缺血再灌注肾损伤。在 2 天内即可观察到肾功能衰竭的显著改善，与早期旁分泌效应一致。然而，在 7 天后，也发现大量供体细胞整合到修复的肾小管结构中。我们得出结论，输注 SAA 阳性细胞可促进急性肾衰竭后的肾脏恢复，并为肾衰竭提供一种潜在的强大而新颖的治疗方法。
