Yang A, Zhang S, Liu Sh, Zhao Q, Pan G
Institute of Tobacco Sciences, Chinese Academy of Agricultural Sciences, Laoshan District, Qingdao, Shandong 266101, China.
Genetika. 2010 Apr;46(4):458-63.
A novel 407 bp nucleotide sequence NTPp13 was isolated from tobacco (Nicotiana tabacum L.) by PCR, its structure and function were characterized. The NTPp13 sequence was highly homologous with the pollen-specific expression promoter Zm13 from maize (Zea mays L.) and contained some key motifs which controlled pollen-specific expression. The NTPp13 was fused to the beta-glucuronidase (GUS) reporter gene and transferred into tobacco. Analysis of the transgenic plants revealed that this putative promoter fragment was sufficient to direct GUS expression specifically in the anther, exactly in the pollen and pollen tube, and that GUS activity reached the maximum at the stage of pollen grain began to separate. Further study showed that the expression of NTPp13 sequence at pollen was stable at the range of temperature measured. These data suggested that the NTPp13 sequence was likely the essential element of promoter region of an unknown pollen-specific gene from tobacco.
通过PCR从烟草（Nicotiana tabacum L.）中分离出一个新的407 bp核苷酸序列NTPp13，并对其结构和功能进行了表征。NTPp13序列与玉米（Zea mays L.）的花粉特异性表达启动子Zm13高度同源，且包含一些控制花粉特异性表达的关键基序。将NTPp13与β-葡萄糖醛酸酶（GUS）报告基因融合并转入烟草。对转基因植物的分析表明，这个推定的启动子片段足以指导GUS在花药中特异性表达，确切地说是在花粉和花粉管中表达，并且GUS活性在花粉粒开始分离阶段达到最大值。进一步研究表明，NTPp13序列在花粉中的表达在所测温度范围内是稳定的。这些数据表明，NTPp13序列可能是烟草中一个未知花粉特异性基因启动子区域的必需元件。
