Aberg B, Edström S, Johannesson A, Larkö O
Department of Otorhinolaryngology, University of Göteborg, Sweden.
Am J Otol. 1991 Mar;12(2):93-6.
Whether cholesteatoma development in chronic otitis media may be part of a generalized hyperproliferation of the epidermis was examined. The renewal time of the stratum corneum was determined in 24 patients presenting with chronic middle ear disease with cholesteatoma. These patients were compared with two control groups: one with chronic otitis media without cholesteatoma and one with otosclerosis. Dansyl chloride was applied to the back of the shoulder beneath occlusive patches for 2 days and the time it took the fluorescing label under ultraviolet light to disappear was measured. The depth of stratum corneum was determined with a standardized tape by measuring the number of strippings until no remaining fluorescing label was visible. This was also analysed with punch biopsies and fluorescence microscopy. However the cholesteatoma patients demonstrated no significant alterations in any of the studied parameters in comparison with the control groups. Based on our techniques and the data obtained there is no evidence of an epidermal hyperproliferative predisposition in patients with cholesteatoma.
研究了慢性中耳炎中胆脂瘤的发展是否可能是表皮普遍过度增殖的一部分。测定了24例患有胆脂瘤的慢性中耳疾病患者角质层的更新时间。将这些患者与两个对照组进行比较：一个是无胆脂瘤的慢性中耳炎患者，另一个是耳硬化症患者。将丹磺酰氯涂于肩部后方封闭贴片下2天，并测量在紫外线下荧光标记消失所需的时间。用标准胶带通过测量剥离次数来确定角质层深度，直到看不到剩余的荧光标记。也通过打孔活检和荧光显微镜进行分析。然而，与对照组相比，胆脂瘤患者在任何研究参数中均未表现出显著改变。基于我们的技术和获得的数据，没有证据表明胆脂瘤患者存在表皮过度增殖倾向。
