Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense M, Denmark.
Expert Rev Proteomics. 2010 Jun;7(3):439-56. doi: 10.1586/epr.10.19.
Recent developments in phosphoproteomic sample-preparation techniques and sensitive mass spectrometry instrumentation have led to large-scale identifications of phosphoproteins and phosphorylation sites from highly complex samples. This has facilitated the implementation of different quantitation strategies in order to study the biological role of protein phosphorylation during disease progression, differentiation or during external stimulation of a cellular system. In this article, a brief summary of the most popular strategies for phosphoproteomic studies is given; however, the main focus will be on different quantitation strategies. Methods for metabolic labeling, chemical modification and label-free quantitation and their applicability or inapplicability in phosphoproteomic studies are discussed.
近年来，磷酸化蛋白质组学样品制备技术和高灵敏度质谱仪器的发展，使得从高度复杂的样本中大规模鉴定磷酸化蛋白质和磷酸化位点成为可能。这为研究疾病进展、分化过程中或细胞系统外部刺激时蛋白质磷酸化的生物学作用，实施不同的定量策略提供了便利。本文简要总结了磷酸化蛋白质组学研究中最常用的策略，重点介绍了不同的定量策略。讨论了代谢标记、化学修饰和无标记定量的方法，以及它们在磷酸化蛋白质组学研究中的适用性或不适用性。
