一种用于 A 型肉毒梭菌神经毒素基因簇分型的新型多重 PCR 方法。
A novel multiplex PCR method for Clostridium botulinum neurotoxin type A gene cluster typing.
机构信息
Department of Microbiology, Osaka City Institute of Public Health and Environmental Sciences, Tennoji-ku, Osaka, Japan.
出版信息
Microbiol Immunol. 2010 May;54(5):308-12. doi: 10.1111/j.1348-0421.2010.00213.x.
A rapid, simple and sensitive multiplex PCR method for boNT/A gene cluster typing was developed by combining the results of BoNT/A subtype (boNT/A1 or /A2) gene detection with ha33 and/or p47 gene detection. Ten isolates associated with infant botulism in Japan were examined and divided into boNT/A gene cluster types 2 and 3 by origin (honey feeding or not) and period (1986-1987 or 1999-2007). It is suggested that this multiplex PCR method will be be useful for epidemiological studies of botulism.
建立了一种快速、简单、灵敏的多重 PCR 方法，用于 BoNT/A 基因簇分型，该方法将 BoNT/A 亚型（boNT/A1 或 /A2）基因检测结果与 ha33 和/或 p47 基因检测结果相结合。对与日本婴儿肉毒中毒相关的 10 株分离株进行了检测，根据来源（是否喂食蜂蜜）和时期（1986-1987 年或 1999-2007 年）将其分为 BoNT/A 基因簇类型 2 和 3。该多重 PCR 方法有望用于肉毒中毒的流行病学研究。
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